Rev. ed. of: Principles of gene manipulation. 6th ed. 2001 and Principles of genome analysis and genomics / Sandy B. Primrose, Richard M. Twyman. 3rd ed. 2003.
Gene manipulation in the post-genomics era
Basic techniques
Cutting and joining DNA molecules
Basic biology of plasmid and phage vectors
Cosmids, phasmids, and other advanced vectors
Gene cloning strategies
Sequencing genes and short stretches of DNA
Changing genes : site-directed mutagenesis and protein engineering
Bioinformatics
Cloning in bacteria other than Escherichia coli
Cloning in Saccharomyces cerevisiaea and other fungi
Gene transfer to animal cells
Genetic manipulation of animals
Gene transfer to plants
Advanced transgenic technology
The organization and structure of genomes
Mapping and sequencing genomes
Comparative genomics
Large-scale mutagenesis and interference
Analysis of the transcriptome
Proteomics I : expression analysis and characterization of proteins
Proteomics II : analysis of protein structures
Proteomics III : protein interactions
Metabolomics and global biochemical networks
Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci
Applications of recombinant DNA technology.
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Principles of gene manipulation and genomics by S. B. Primrose. ISBN 1405135441. Published by Blackwell in 2006. Publication and catalogue information, links to buy online and reader comments.
