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Culture of animal cells : a manual of basic technique and specialized applications
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Culture of animal cells : a manual of basic technique and specialized applications

R. Ian Freshney

Publication Data

Contents

Proposed TOC

1. Introduction

2. Biology of Cultured Cells

3. Designing and Layout

4. Equipment & Materials

5. Aseptic Technique

6. Safety & Bioethics

7. Culture Vessels & Substrates

8. Media & Supplements

9. Serum-free media

10. Preparation & Sterilization

11. Primary Culture

12. Subculture & Cell Lines

13. Authentication & Validation

13.1.Misidentification & Cross-contamination

13.2.Authentication Procedures

13.3.Sourcing Cell Lines

13.4.Maintenance and Storage Procedures

14. loning & Selection

15. Cell Separation

16. Characterization

17. Differentiation

18. Transformation & Immortalization

19. Microbial Contamination

20. Quantitation

21. Viability & Cytotoxicity Assays

22. Culture of Specific Cell Types, Background & Principles
22.1.Adult-derived Cells
22.2.Stem Cells
22.3.Tumor Cells
22.4.Non-human Animal Cells

23. Three-dimensional Culture

24. Scale-up & Automation

25. Applications
25.1.Tissue Engineering & Regenerative Medicine
25.2.Production of Biopharmaceuticals
25.3.Drug Screening
25.3.1. Toxicity
25.3.2. Functional Targeting

26. Training Programs
27. Problem Solving

Appendices:

Calculations & conversions

Glossary

General Textbooks

References

Index

Online:

Specialized Cell Culture Protocols (e.g. Mammary epithelial cells)

Specialized Techniques Protocols (e.g. Autoradiography, Chromosome Analysys)

Sources & Suppliers of Equipment & Materials

Database of Misidentified Cell Lines

Topics

Catalogue Data

ISBD

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Culture of animal cells : a manual of basic technique and specialized applications by R. Ian Freshney. ISBN 9781118873649. Published by Wiley Blackwell in 2015. Publication and catalogue information, links to buy online and reader comments.

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